Tentative identification of RNA‐dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases

  title={Tentative identification of RNA‐dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases},
  author={E. V. Koonin and A E Gorbalenya and Konstantin Chumakov},
  journal={FEBS Letters},

Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences.

It is hypothesized that all positive-strand RNA viruses and some related double-stranded RNA viruses could have evolved from a common ancestor virus that contained genes for RNA-dependent RNA polymerase, a chymotrypsin-related protease that also functioned as the capsid protein, and possibly an RNA helicase.

The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses.

  • E. Koonin
  • Biology
    The Journal of general virology
  • 1991
Phylogenetic analysis using three independent methods of three construction confirmed the separation of the positive-strand RNA viral polymerases into three supergroups and revealed some unexpected clusters within the supergroups.

The polymerase subunit of a dsRNA virus plays a central role in the regulation of viral RNA metabolism

It is shown that the enzyme is also able to use dsRNA templates to perform semi‐conservative RNA transcription in vitro without the assistance of other proteins, suggesting a model explaining important aspects of viral RNA metabolism regulation in terms of enzymatic properties of the polymerase subunit.

Characterization of φ12, a Bacteriophage Related to φ6: Nucleotide Sequence of the Large Double-Stranded RNA

Comparing the genomic sequences of these viruses has allowed evaluation of important genetic as well as structural motifs and are resulting in greater understanding of viral evolution and the role played by genetic and structural variation in the assembly mechanisms of the cystoviruses.

Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses

Results reveal that the non-virion, intracellular RNA-replication complexes of some positive-strand RNA viruses share parallels with the structure, assembly and function of the replicative cores of extracellular virions of reverse-transcribing viruses and double-stranded RNA viruses.

Replicase activity of purified recombinant protein P2 of double‐stranded RNA bacteriophage φ6

Rec recombinant protein P2 of double‐stranded RNA bacteriophage φ6 was purified and demonstrated in an in vitro enzymatic assay to act as the replicase to show template‐dependent RNA synthesis directly with an isolated putative polymerase protein.

A structural and primary sequence comparison of the viral RNA-dependent RNA polymerases.

A systematic bioinformatic approach to identifying the evolutionarily conserved regions of proteins has verified the universality of a newly described conserved motif in RNA-dependent RNA polymerases (motif F), which is the N-terminal portion of motif F.

Comparison of Polymerase Subunits from Double-Stranded RNA Bacteriophages

Rec recombinant Pol subunits from the φ6-related bacteriophages φ8 and φ13 are purified and assayed their polymerase activities in vitro, suggesting that RNA transcription in Cystoviridae is controlled by the template specificity of the polymerase subunit.

Characterization of Φ8, a Bacteriophage Containing Three Double-Stranded RNA Genomic Segments and Distantly Related to Φ6

The three double-stranded RNA genomic segments of bacteriophage Φ8 were copied as cDNA, and their nucleotide sequences were determined. Although the organization of the genome is similar to that of

Self-assembly of double-stranded RNA bacteriophages.




Birnavirus RNA polymerase is related to polymerases of positive strand RNA viruses.

Recently the short segment of genomic double-stranded RNA of a birnavirus, infectious bursa dlsease virus (IBDV), was sequenced and it was claimed that its encoded product RNA polymereae had no

Evidence for genetic relationship between RNA and DNA viruses from the sequence homology of a putative polymerase gene of bluetongue virus with that of vaccinia virus: conservation of RNA polymerase genes from diverse species.

Segment 1 of the double stranded RNA genome of bluetongue virus serotype 10, encoding the largest viral core protein, VP1, has been determined and extensive homology with the vaccinia virus 147K Da DNA-directed RNA polymerase subunit is revealed.

Identification of a putative RNA dependent RNA polymerase encoded by a yeast double stranded RNA virus

A partial (carboxy terminal) sequence for a putative RDRP from the yeast virus ScV is reported, the first RDRPs from a double stranded RNA virus of eucaryotes.

Viral RNA polymerases.

This review shows that the viral RNA polymerases are complex in both structure and function, being composed of multiple subunits and carrying multiple functions.

Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase

This report completes the sequence analysis of phi 6 and determines the nucleotide sequence of cDNA derived from the largest RNA segment (L), which constitutes the first complete sequence of a double-stranded RNA genome virus.