Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension.

@article{Zhelev1993TensionstabilizedPI,
  title={Tension-stabilized pores in giant vesicles: determination of pore size and pore line tension.},
  author={Doncho V. Zhelev and Dave Needham},
  journal={Biochimica et biophysica acta},
  year={1993},
  volume={1147 1},
  pages={
          89-104
        }
}
We present the first observations of giant, long-existing, stabilized pores in vesicle membranes. Using a new experimental technique for studying the electro-permeabilization of lipid membranes, giant liposomes (from 25 to 56 microns in diameter) were subjected to single, square, electric pulses (duration 150 microseconds and electric field strength from 63 to 126 kV/m). The liposomes were held by a micropipet and small membrane tensions were created by controlling the pipet suction pressure… CONTINUE READING

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