Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction.

@article{Mifune2013TendonGR,
  title={Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction.},
  author={Yutaka Mifune and Tomoyuki Matsumoto and Koji Takayama and Satoshi Terada and Naosumi Sekiya and Ryosuke Kuroda and Masahiro Kurosaka and F. Fu and Johnny Huard},
  journal={Biomaterials},
  year={2013},
  volume={34 22},
  pages={5476-87}
}
As a new strategy for enhancing recovery after anterior cruciate ligament (ACL) reconstruction, we developed a cell sheet wrapped graft using ACL-derived CD34+ cells. Our results from an ex vivo cell migration assay demonstrated gradual cell migration into the graft from the cell sheet, and an enzyme-linked immunosorbent assay indicated that the cell sheet cultures secreted a significantly greater amount of VEGF than typical monolayer cultures. In the in vivo study, the rats received one of… CONTINUE READING