Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.

@article{Wightman1991TemporallyRC,
  title={Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.},
  author={R. Wightman and J. A. Jankowski and R. Kennedy and K. T. Kawagoe and T. J. Schroeder and D. J. Leszczyszyn and J. A. Near and E. Diliberto and O. H. Viveros},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 23},
  pages={
          10754-8
        }
}
Secretion of catecholamines from single bovine chromaffin cells in culture was elicited by brief pressure ejections from a micropipette containing nicotine, carbamoylcholine, or potassium ions or by mechanical stimulation. Release was monitored electrochemically with a carbon-fiber microelectrode placed adjacent to the cell. Cyclic voltammetry was used to identify secreted species, whereas constant potential amperometry was used for improved temporal resolution (millisecond range) of… Expand
Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode.
TLDR
An efficient method is reported where the same electrochemical microelectrode is used to electropermeabilize an adjacent chromaffin cell and then measure the consequent quantal catecholamine release using amperometry, allowing precise timing of the stimulus, stimulation of a single cell at a time, and can be used to load membrane-impermeant substances into a cell. Expand
Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells
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Under voltage-clamp conditions, stochastically occurring signals can be recorded from adrenal chromaffin cells using a carbon-fibre electrode as an electrochemical detector and identifies a pedestal or 'foot' at the onset of unitary events which may represent the slow leak of catecholamine molecules out of a narrow 'fusion pore' before the pore dilates for complete exocytosis. Expand
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TLDR
The data suggest that the rate-limiting step for chemical secretion from adrenal medullary cells during exocytosis is the dissociation of catecholamines from the vesicular matrix at the surface of the cell. Expand
Quantal secretion of catecholamines measured from individual bovine adrenal medullary cells permeabilized with digitonin.
TLDR
Secretion of catecholamines from individual bovine adrenal medullary cells grown in primary culture has been investigated with a carbon-fiber microelectrode placed adjacent to the cells, and the contents of individual vesicles are detected in the form of sharp spikes. Expand
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An electrochemical microelectrode located immediately adjacent to a single neuroendocrine cell can record spikes of amperometric current that result from quantal exocytosis of oxidizable transmitterExpand
No . 7 F TIME-COURSE OF RELEASE OF CATECHOLAMINES FROM INDIVIDUAL VESICLES DURING EXOCYTOSIS AT ADRENAL MEDULLARY CELLS
The time codrse of extrusion of the vesicular contents during exocytosis has been examined at adrenal medullary cells with carbon-fiber microelectrodes. Two electrochemical techniques were employed:Expand
Real-time monitoring of chemical transmission in slices of the murine adrenal gland.
TLDR
Bright-field and immunofluorescent microscopy supported that chromaffin cells in the adrenal medulla are organized into clusters and positively stain for tyrosine hydroxylase confirming that they are catecholaminergic. Expand
Chaos evidence in catecholamine secretion at chromaffin cells
Abstract Chromaffin cells secrete catecholamine molecules via exocytosis process. Each exocytotic event is characterized by a current spike, which corresponds to the amount of released catecholamineExpand
Comparison of Cytosolic Ca 2 + and Exocytosis Responses from Single Rat and Bovine Chromaffin Cells
The relationship between cytosolic Ca and catecholamine secretion during stimulussecretion coupling has been examined at individual chromaffin cells isolated from the cow and rat. VesicularExpand
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Inhibition of exocytosis by intracellularly applied antibodies against a chromaffin granule-binding protein
TLDR
It is shown, by two independent techniques in two different cell types, that antibodies against this protein inhibit exocytosis, a plasma membrane protein with high affinity for secretory vesicles that has been isolated from bovine adrenal chromaffin cells. Expand
Bevelled carbon-fiber ultramicroelectrodes
Abstract A technique for polishing the surface of carbon-fiber ultramicroelectrodes to a bevelled tip is described which simplifies electrode preparation. The bevelled surface has an active area thatExpand
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