• Corpus ID: 9381789

Temporal release of growth factors from platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis.

@article{McLellan2014TemporalRO,
  title={Temporal release of growth factors from platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis.},
  author={Jonathan McLellan and Sarah Plevin},
  journal={International Journal of Applied Research in Veterinary Medicine},
  year={2014},
  volume={12},
  pages={44-53}
}
  • J. McLellan, S. Plevin
  • Published 2014
  • Medicine, Biology
  • International Journal of Applied Research in Veterinary Medicine
Objectives: To evaluate platelet-rich fibrin (PRF) in horses and compare temporal release of growth factors (GF) to platelet-rich 

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References

SHOWING 1-10 OF 35 REFERENCES
Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies
TLDR
Choukroun's platelet-rich fibrin (PRF) is a second generation platelet concentrate that releases high quantities of three main growth factors, including TGFβ-1, platelet derived growth factor AB, PDGF-AB and an important coagulation matricellular glycoprotein during 7 days.
Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing.
  • J. ChoukrounA. Diss D. Dohan
  • Medicine, Biology
    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
  • 2006
Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots: a comparative in vitro analysis.
TLDR
Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC.
Platelet Rich Plasma: Biology and New Technology
TLDR
Fundamental background on platelet biology and the role of platelets in both hemostasis and wound healing, as well as methods of preparing, characterizing, and using platelet rich plasma are presented to provide the reader a foundation on which to critically evaluate prior studies and plan future work are presented.
Effects of platelet-derived growth factor-BB on the metabolic function and morphologic features of equine tendon in explant culture.
TLDR
Results suggest that rhPDGF-BB use may be of benefit in the repair of equine tendon, particularly through induction of collagen type I mRNA.
Effects of activated platelet concentrates on human primary cultures of fibroblasts and osteoblasts.
TLDR
There were no statistical differences in fibroblast proliferation, or in osteoblast growth and functions between serum-free conditions and the platelet gel treatment, therefore, neither fibro Blast proliferation nor osteoblasts growth and function were affected by the activated platelet concentrates in vitro.
Effects of platelet-rich plasma on the repair of wounds on the distal aspect of the forelimb in horses.
TLDR
Platelet-rich plasma favored excessive development of granulation tissue and significantly slowed wound healing at 1, 2, and 3 weeks after surgery, which may better suit wounds with massive tissue loss or chronic wounds that would benefit from a fresh source of mediators to accelerate the healing process.
A proposed protocol for the standardized preparation of PRF membranes for clinical use.
Autologous platelet‐rich fibrin matrix as cell therapy in the healing of chronic lower‐extremity ulcers
TLDR
From the results of this small‐scale pilot study, PRFM shows significant potential for closing of chronic leg ulcers, and patients with >50% reduction in wound area were allowed to continue to complete closure.
...
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