• Corpus ID: 9381789

Temporal release of growth factors from platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis.

  title={Temporal release of growth factors from platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) in the horse: a comparative in vitro analysis.},
  author={Jonathan McLellan and Sarah Plevin},
  journal={International Journal of Applied Research in Veterinary Medicine},
  • J. McLellan, S. Plevin
  • Published 2014
  • Medicine, Biology
  • International Journal of Applied Research in Veterinary Medicine
Objectives: To evaluate platelet-rich fibrin (PRF) in horses and compare temporal release of growth factors (GF) to platelet-rich 

Figures and Tables from this paper

The L-PRF Membrane (Fibrin Rich in Platelets and Leukocytes) and Its Derivatives (A-PRF, I-PRF) Are Useful as a Source of Stem Cells in Regenerative Wound Therapy: Experimental Work on the Horse
To ensure that tissue engineering methods are widely applicable in the clinical setting, it is necessary to modify them in such a way that they are readily available and relatively easy to use in daily clinical routine.
Production Protocol Standardisation, Macroscopic and Histological Evaluation, and Growth Factor Quantification of Canine Leukocyte-and Platelet-Rich Fibrin Membranes
The data confirm the effectiveness of this protocol and of Wound Box to produce L-PRF membranes in dogs and confirm a well-defined histoarchitecture with five layers reproducing density and distribution of blood cells in this biomaterial.
Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma?
Although the double-centrifugation protocol caused premature platelet aggregation, it seems to be an effective method for preparation of PRP with high platelet and TGF-β1 concentrations.
Fibrin Rich in Leukocyte-Platelets ( L-PRF ) and Injectable Fibrin Rich Platelets ( i-PRF ) , Two Opportunity in Regenerative Surgery : Review of The Sciences and Literature
This review article focuses on the development of various platelet concentrates, their fabrication procedure, advantages and disadvantages for use in regenerative surgery and healing process.
Platelet-Rich Products and Their Application to Osteoarthritis.
A new instrument aid of plastic surgeon : membranes L-PRF ( Platelet-Rich-Fibrin )
Exogenous adding of Platelets Rich Plasma (PRP) on wound’s site not only speeds up physiological healing but also provides an additional substrate to tie up for others cells such as endothelial and smooth muscle ones, fibroblasts, leucocytes, keratinocytes and stem cells as well as platelets.
Selection of an Animal Model for the Production of Leukocyte-Fibrin Rich Platelet Membranes (L-PRF); Standardized Protocol Proposed for Clinical Use and the Use of L-PRF Wound Box®
The hypothesis is that the L-PRF is easy to produce in the horse, without modifications of the human protocol, thus allowing a better standardization of thehuman protocol.


Slow release of growth factors and thrombospondin-1 in Choukroun's platelet-rich fibrin (PRF): a gold standard to achieve for all surgical platelet concentrates technologies
Choukroun's platelet-rich fibrin (PRF) is a second generation platelet concentrate that releases high quantities of three main growth factors, including TGFβ-1, platelet derived growth factor AB, PDGF-AB and an important coagulation matricellular glycoprotein during 7 days.
Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing.
  • J. Choukroun, A. Diss, D. Dohan
  • Medicine, Biology
    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
  • 2006
Platelet-rich fibrin constructs elute higher concentrations of transforming growth factor-β1 and increase tendon cell proliferation over time when compared to blood clots: a comparative in vitro analysis.
Both PRFM constructs are comprised of a dense fibrin scaffold that contains increased concentrations of TGF-β1 and are capable of increasing tendon cell proliferation over time when compared with a BC.
Bacteria‐induced release of white cell‐ and platelet‐derived vascular endothelial growth factor in vitro
In vitro release of VEGF from white blood cells and platelets stimulated by bacterial antigens and supernatants from stored red cell components is studied for poor prognosis after resection of primary colorectal cancer.
Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel
The results indicated that P-PRP obtained by a manual method was affected by intrinsic factors such as the breed, gender and age, and should be aware that cellular and GF release from P- PRP/P-PRG could change according with the intrinsic variables associated with a patient in particular.
Leukocyte- and platelet-rich fibrin (L-PRF) for long-term delivery of growth factor in rotator cuff repair: review, preliminary results and future directions.
L-PRF in its optimal standard/gelatinous-type matrix can store and deliver locally specific healing growth factors for up to 28 days and may be a useful adjunct in rotator cuff repair.
Platelet Rich Plasma: Biology and New Technology
Fundamental background on platelet biology and the role of platelets in both hemostasis and wound healing, as well as methods of preparing, characterizing, and using platelet rich plasma are presented to provide the reader a foundation on which to critically evaluate prior studies and plan future work are presented.
Effects of platelet-derived growth factor-BB on the metabolic function and morphologic features of equine tendon in explant culture.
Results suggest that rhPDGF-BB use may be of benefit in the repair of equine tendon, particularly through induction of collagen type I mRNA.
Effects of activated platelet concentrates on human primary cultures of fibroblasts and osteoblasts.
There were no statistical differences in fibroblast proliferation, or in osteoblast growth and functions between serum-free conditions and the platelet gel treatment, therefore, neither fibro Blast proliferation nor osteoblasts growth and function were affected by the activated platelet concentrates in vitro.