Temporal regulation of gene expression of the Escherichia coli bacteriophage phiEco32.

@article{Pavlova2012TemporalRO,
  title={Temporal regulation of gene expression of the Escherichia coli bacteriophage phiEco32.},
  author={Olga S Pavlova and Daria G. Lavysh and Evgeny Klimuk and Marko Djordjevic and Dmitry A. Ravcheev and Mikhail S. Gelfand and Konstantin Severinov and Natalja Akulenko},
  journal={Journal of molecular biology},
  year={2012},
  volume={416 3},
  pages={389-99}
}
Escherichia coli phage phiEco32 encodes two proteins that bind to host RNA polymerase (RNAP): gp79, a novel protein, and gp36, a distant homolog of σ(70) family proteins. Here, we investigated the temporal pattern of phiEco32 and host gene expression during infection. Host transcription shutoff and three distinct bacteriophage temporal gene classes (early, middle, and late) were revealed. A combination of bioinformatic and biochemical approaches allowed identification of phage promoters… CONTINUE READING

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