Temporal dynamics of prokaryotic communities in the marine sponge Sarcotragus spinosulus.

@article{Hardoim2014TemporalDO,
  title={Temporal dynamics of prokaryotic communities in the marine sponge Sarcotragus spinosulus.},
  author={Cristiane Cassiolato Pires Hardoim and Rodrigo Costa},
  journal={Molecular ecology},
  year={2014},
  volume={23 12},
  pages={
          3097-112
        }
}
In spite of their putative relevance to host functioning, in-depth knowledge of sponge microbiome stability over time is scarce. This study tackles the temporal maintenance of bacterial and archaeal assemblages in the model host Sarcotragus spinosulus along three successive years. Prokaryotic communities were profiled by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 454-pyrosequencing of S. spinosulus-derived 16S rRNA gene amplicons. Prevailing bacterial phyla… CONTINUE READING
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