Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes.

@article{Wu1996TemporalDI,
  title={Temporal distinctions in the synthesis and accumulation of proteins by oocytes and cumulus cells during maturation in vitro of bovine oocytes.},
  author={Ben-chuan Wu and George G Ignotz and W Bruce Currie and Xiangzhong Yang},
  journal={Molecular reproduction and development},
  year={1996},
  volume={45 4},
  pages={560-5}
}
Successful in vitro maturation (IVM) of bovine oocytes requires continual and/or episodic protein synthesis by cumulus-oocyte complexes. This study was designed to expose time-dependent changes in protein synthesis and accumulation by bovine oocytes and cumulus cells during routine IVM. Silver staining after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated little if any change in protein species present or their relative contents in oocytes during IVM; one… CONTINUE READING

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