Temporal and spatial properties of cellular Ca2+ flux in trout ventricular myocytes.

@article{Shiels2005TemporalAS,
  title={Temporal and spatial properties of cellular Ca2+ flux in trout ventricular myocytes.},
  author={Holly A Shiels and Ed White},
  journal={American journal of physiology. Regulatory, integrative and comparative physiology},
  year={2005},
  volume={288 6},
  pages={R1756-66}
}
Confocal microscopy was used to investigate the temporal and spatial properties of Ca(2+) transients and Ca(2+) sparks in ventricular myocytes of the rainbow trout (Oncorhynchus mykiss). Confocal imaging confirmed the absence of T tubules and the long ( approximately 160 microm), thin ( approximately 8 microm) morphology of trout myocytes. Line scan imaging of Ca(2+) transients evoked by electrical stimulation in cells loaded with fluo 4 revealed spatial inhomogeneities in the temporal… CONTINUE READING