Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding.

@article{Bleck2014TemporalAS,
  title={Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding.},
  author={Marina Bleck and Michelle S. Itano and Daniel S Johnson and Vinoo Thomas and Alison J. North and Paul D Bieniasz and Sanford M Simon},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2014},
  volume={111 33},
  pages={12211-6}
}
HIV-1 virions assemble at the plasma membrane of mammalian cells and recruit the endosomal sorting complex required for transport (ESCRT) machinery to enable particle release. However, little is known about the temporal and spatial organization of ESCRT protein recruitment. Using multiple-color live-cell total internal reflection fluorescence microscopy, we observed that the ESCRT-I protein Tsg101 is recruited together with Gag to the sites of HIV-1 assembly, whereas later-acting ESCRT proteins… CONTINUE READING