Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site.

@article{KoyamaHonda2013TemporalAO,
  title={Temporal analysis of recruitment of mammalian ATG proteins to the autophagosome formation site.},
  author={Ikuko Koyama-Honda and Eisuke Itakura and Takahiro K Fujiwara and Noboru Mizushima},
  journal={Autophagy},
  year={2013},
  volume={9 10},
  pages={1491-9}
}
Autophagosome formation is governed by sequential functions of autophagy-related (ATG) proteins. Although their genetic hierarchy in terms of localization to the autophagosome formation site has been determined, their temporal relationships remain largely unknown. In this study, we comprehensively analyzed the recruitment of mammalian ATG proteins to the autophagosome formation site by live-cell imaging, and determined their temporal relationships. Although ULK1 and ATG5 are separated in the… CONTINUE READING