Temporal Divergence in the Pattern of Messenger RNA Expression in Bovine Embryos Cultured from the Zygote to Blastocyst Stage In Vitro or In Vivo1

@inproceedings{Lonergan2003TemporalDI,
  title={Temporal Divergence in the Pattern of Messenger RNA Expression in Bovine Embryos Cultured from the Zygote to Blastocyst Stage In Vitro or In Vivo1},
  author={Pat Lonergan and Dimitrios Rizos and Alfonso Guti{\'e}rrez-Ad{\'a}n and Pedro Miguel Lopes Moreira and Belén Pintado and Julio de la Fuente and Maurice P. Boland},
  booktitle={Biology of reproduction},
  year={2003}
}
Abstract The objective of this study was to examine the time during the postfertilization period that gene expression patterns in in vitro-cultured bovine embryos diverge from those of their in vivo-cultured counterparts. Presumptive bovine zygotes were produced by in vitro maturation and fertilization of immature oocytes collected from the ovaries of slaughtered animals. Approximately 20 h post insemination (hpi), zygotes were denuded and randomly divided into two groups for culture either in… Expand
A comparative expression analysis of gene transcripts in post-fertilization developmental stages of bovine embryos produced in vitro or in vivo.
TLDR
Temporal variation in the relative abundance of transcripts during the post-fertilization developmental stages of in vitro and in vivo embryos may reflect the effect of the in vitro culture condition on the transcriptional activity thereby on the developmental competence of the resulting embryos. Expand
Temporal expression of transcripts related to embryo quality in bovine embryos cultured from the two‐cell to blastocyst stage in vitro or in vivo
TLDR
Analysis of relative transcript abundance for FOXO3A, EEF1G, HMG2, and REA indicated that these transcripts are most likely of maternal origin produced in the oocyte during growth and final maturation. Expand
The effect of long-term in vivo culture in bovine oviduct and uterus on the development and cryo-tolerance of in vitro produced bovine embryos.
TLDR
Tubal access is a promising tool to provide a further basis for studying embryo sensitivity to environmental changes and the duration of in vivo culture crucially influenced the cryo-tolerance of produced blastocysts. Expand
Development and pattern of mRNA relative abundance of bovine embryos cultured in the isolated mouse oviduct in organ culture
TLDR
Data confirm that the IMO is capable of supporting development of bovine embryos and the pattern of transcript abundance in embryos derived from the IMo is similar to that of in vivo derived embryos. Expand
Expression pattern of oxygen and stress-responsive gene transcripts at various developmental stages of in vitro and in vivo preimplantation bovine embryos.
TLDR
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Analysis of gene transcription alterations at the blastocyst stage related to the long-term consequences of in vitro culture in mice.
TLDR
It is suggested that culture-induced alterations in transcription at the blastocyst stage related to epigenetic mechanisms provide a foundation for understanding the molecular origin at the time of preimplantation development of the long-term consequences of IVC in mammals. Expand
Suppression of connexin 43 and E‐cadherin transcripts in in vitro derived bovine embryos following culture in vitro or in vivo in the homologous bovine oviduct
TLDR
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Transcriptome Changes at the Initiation of Elongation in the Bovine Conceptus1
TLDR
The aim of this study was to examine the temporal changes in transcriptional profile as the embryo develops from a spherical blastocyst on Day 7 to an ovoid conceptus at the initiation of elongation on Day 13 and to highlight differences in these temporal gene expression dynamics between in vivo- and in vitro-derived blastocysts that may be associated with embryonic survival/mortality using the bovine Affymetrix microarray. Expand
Culture of bovine embryos in intermediate host oviducts with emphasis on the isolated mouse oviduct.
TLDR
This review focuses on the use of oviducts in vitro or in vivo as intermediate hosts for postfertilization culture environment of bovine in vitro-produced zygotes with emphasis on the mouse model. Expand
Embryonic gene activation in in vitro produced embryos of the domestic cat (Felis catus).
TLDR
The results showed an increase in transcript abundance from the matured oocyte to the 2-cell embryo for all examined genes except for IGF2R, indicating that, in vitro, the embryonic genome is activated shortly after fertilization. Expand
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