Temporal ChIP-on-Chip of RNA-Polymerase-II to detect novel gene activation events during photoreceptor maturation

@inproceedings{Tummala2010TemporalCO,
  title={Temporal ChIP-on-Chip of RNA-Polymerase-II to detect novel gene activation events during photoreceptor maturation},
  author={Padmaja Tummala and Raghuveer Singh Mali and Eduardo Guzm{\'a}n and Xiao Zhang and Kenneth P. Mitton},
  booktitle={Molecular vision},
  year={2010}
}
PURPOSE During retinal development, post-mitotic neural progenitor cells must activate thousands of genes to complete synaptogenesis and terminal maturation. While many of these genes are known, others remain beyond the sensitivity of expression microarray analysis. Some of these elusive gene activation events can be detected by mapping changes in RNA polymerase-II (Pol-II) association around transcription start sites. METHODS High-resolution (35 bp) chromatin immunoprecipitation (ChIP)-on… CONTINUE READING