Template topology and transcription: chromatin templates relaxed by localized linearization are transcriptionally active in yeast.

@article{Liang1997TemplateTA,
  title={Template topology and transcription: chromatin templates relaxed by localized linearization are transcriptionally active in yeast.},
  author={Chih-Chuan Liang and William Garrard},
  journal={Molecular and cellular biology},
  year={1997},
  volume={17 5},
  pages={2825-34}
}
To address the role of transient torsional stress in transcription, we have utilized the regulated expression of HO endonuclease in yeast to create double-strand breaks in DNA templates in vivo at preselected sites. Linearization of circular minichromosomes, either 2 kb upstream or immediately downstream of a lacZ reporter gene controlled by the yeast… CONTINUE READING