Template-switching during DNA synthesis by Thermus aquaticus DNA polymerase I.

@article{Odelberg1995TemplateswitchingDD,
  title={Template-switching during DNA synthesis by Thermus aquaticus DNA polymerase I.},
  author={Shannon Odelberg and Robert B Weiss and Akiko Hata and R. White},
  journal={Nucleic acids research},
  year={1995},
  volume={23 11},
  pages={2049-57}
}
Recombinant DNA molecules are often generated during the polymerase chain reaction (PCR) when partially homologous templates are available [e.g., see Pääbo et al. (1990) J. Biol. Chem. 265, 4718-4721]. It has been suggested that these recombinant molecules are a consequence of truncated extension products annealing to partially homologous templates on subsequent PCR cycles. However, we demonstrate here that recombinants can be generated during a single round of primer extension in the absence… CONTINUE READING