Template requirement and initiation site selection by hepatitis C virus polymerase on a minimal viral RNA template.

Abstract

RNA-dependent RNA polymerase, NS5B protein, catalyzes replication of viral genomic RNA, which presumably initiates from the 3'-end. We have previously shown that NS5B can utilize the 3'-end 98-nucleotide (nt) X region of the hepatitis C virus (HCV) genome as a minimal authentic template. In this study, we used this RNA to characterize the mechanism of RNA… (More)

Topics

  • Presentations referencing similar topics