Template-primer analogs as substrates for DNA polymerase.

  title={Template-primer analogs as substrates for DNA polymerase.},
  author={Thomas R. Webb and Parkash Jhurani and P. G. Ng},
  journal={Nucleic acids research},
  volume={15 10},
In order to gain more understanding about the mode of action of DNA polymerase, eight related partially self-complementary "hairpin" shaped oligodeoxynucleotides were prepared. Four of the oligomers contained either 1-beta-D-arabinofuranosyluracil (ara-U) or 1-beta-D-2'deoxyxylofuranosylthymine (dxT) nucleoside analogs at their 3' termini. We investigated the ability of the oligomers to prime DNA synthesis in relationship to the stability of the hybridized region, the nature of the sugar… CONTINUE READING