Template length, sequence context, and 3'-5' exonuclease activity modulate replicative bypass of thymine glycol lesions in vitro.

@article{Clark1989TemplateLS,
  title={Template length, sequence context, and 3'-5' exonuclease activity modulate replicative bypass of thymine glycol lesions in vitro.},
  author={J. Marshall Clark and G. Peter Beardsley},
  journal={Biochemistry},
  year={1989},
  volume={28 2},
  pages={775-9}
}
cis-Thymine glycol, a product of ionizing radiation damage to DNA, has been introduced quantitatively at a single site into oligonucleotide templates. The ability of DNA polymerases to replicate templates containing thymine glycol was studied by a primer extension assay, and three factors that influence replicative bypass of this lesion in vitro have been identified. These factors include template length, sequence context, and 3'-5' exonuclease activity. Synthesis by the large fragment of DNA… CONTINUE READING
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