Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages

Abstract

Up to now, the direct ligation of two DNA fragments with opposite directions to obtain 3'-3' or 5'-5' phosphate ester bonds is still challenging. The only way to obtain DNA oligonucleotides containing a 3'-3' or 5'-5' inversion of polarity sites is based on professional DNA chemical synthesis. Herein, we demonstrate a convenient template-directed chemical… (More)
DOI: 10.1038/srep04595

Topics

4 Figures and Tables

Cite this paper

@inproceedings{Chen2014TemplatedirectedCL, title={Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages}, author={Haodong Chen and Feng Du and Gangyi Chen and Frank Streckenbach and Afshan Yasmeen and Yun Zhao and Zhuo Tang}, booktitle={Scientific reports}, year={2014} }