Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages

@article{Chen2014TemplatedirectedCL,
  title={Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages},
  author={H. Chen and Feng Du and Gangyi Chen and Frank Streckenbach and A. Yasmeen and Y. Zhao and Zhuo Tang},
  journal={Scientific Reports},
  year={2014},
  volume={4}
}
Up to now, the direct ligation of two DNA fragments with opposite directions to obtain 3′-3′ or 5′-5′ phosphate ester bonds is still challenging. The only way to obtain DNA oligonucleotides containing a 3′-3′ or 5′-5′ inversion of polarity sites is based on professional DNA chemical synthesis. Herein, we demonstrate a convenient template-directed chemical ligation that enables 3′-3′ and 5′-5′ linkages of two DNA oligonucleotides. This method is based on the assembly of two oligonucleotides on a… Expand
2 Citations
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