Temperature shift-induced reactive oxygen species enhanced validamycin A production in fermentation of Streptomyces hygroscopicus 5008.

@article{Wei2012TemperatureSR,
  title={Temperature shift-induced reactive oxygen species enhanced validamycin A production in fermentation of Streptomyces hygroscopicus 5008.},
  author={Zhen-hua Wei and Hang Wu and Lin-quan Bai and Zixin Deng and Jian-jiang Zhong},
  journal={Bioprocess and biosystems engineering},
  year={2012},
  volume={35 8},
  pages={1309-16}
}
In order to enhance the production of validamycin A (VAL-A), a widely used agricultural antibiotic, a temperature shift strategy was developed in the fermentation of Streptomyces hygroscopicus 5008. VAL-A production and the transcriptional levels of its structural genes were enhanced in the optimal temperature shift condition. The addition of diphenyleneiodonium [DPI, reactive oxygen species (ROS) inhibitor] inhibited intracellular ROS level and VAL-A production, which indicated that ROS signal… CONTINUE READING