Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene.

@article{Leidich1995TemperaturesensitiveYG,
  title={Temperature-sensitive yeast GPI anchoring mutants gpi2 and gpi3 are defective in the synthesis of N-acetylglucosaminyl phosphatidylinositol. Cloning of the GPI2 gene.},
  author={Steven D. Leidich and Z. T. Kostova and Robert R. Latek and Leslie C. Costello and D A Drapp and William Gray and Jan S. Fassler and Peter Orlean},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 22},
  pages={13029-35}
}
To identify genes required for the synthesis of glycosyl phosphatidylinositol (GPI) membrane anchors in yeast, we devised a way to isolate GPI anchoring mutants in which colonies are screened for defects in [3H]-inositol incorporation into protein. The gpi1 mutant, identified in this way, is temperature sensitive for growth and defective in vitro in the synthesis of GlcNAc-phosphatidylinositol, the first intermediate in GPI biosynthesis (Leidich, S. D., Drapp, D. A., and Orlean, P. (1994) J… CONTINUE READING

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