Temperature-sensitive mutants of p53 associated with human carcinoma of the lung.

@article{Medcalf1992TemperaturesensitiveMO,
  title={Temperature-sensitive mutants of p53 associated with human carcinoma of the lung.},
  author={E A Medcalf and Toshitada Takahashi and Itsuo Chiba and J Hsu Minna and Jonathan Milner},
  journal={Oncogene},
  year={1992},
  volume={7 1},
  pages={71-6}
}
We have compared the effects of specific point mutations on the tertiary and quaternary structure of the human p53 protein. Eight mutants, each derived from primary resected tissues of lung carcinomas, were expressed in vitro under strictly defined conditions, such that the only known variant was the point mutation present in each p53 mRNA. All the mutations were located in highly conserved domains. The tertiary structure of each mutant protein was investigated by reactivity with anti-p53… CONTINUE READING