Temperature-sensitive mutants of hsp82 of the budding yeast Saccharomyces cerevisiae

@article{Kimura2004TemperaturesensitiveMO,
  title={Temperature-sensitive mutants of hsp82 of the budding yeast Saccharomyces cerevisiae},
  author={Yoko Kimura and Seiji Matsumoto and Ichiro Yahara},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={242},
  pages={517-527}
}
The budding yeast Saccharomyces cerevisiae has two HSP90-related genes per haploid genome, HSP82 and HSC82. Random mutations were induced in vitro in the HSP82 gene by treatment of the plasmid with hydroxylamine. Four temperature-sensitive (ts) mutants and one simultaneously is and cold-sensitivie (cs) mutant were then selected in a yeast strain in which HSC82 had previously been disrupted. The mutants were found to have single base changes in the coding region, which caused single amino acid… 
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