Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures

@article{Jahic2003TemperatureLF,
  title={Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures},
  author={Mehmedalija Jahic and Fredrik W{\aa}llberg and Monika Bollok and Percival Garcia and S O Enfors},
  journal={Microbial Cell Factories},
  year={2003},
  volume={2},
  pages={6 - 6}
}
BACKGROUND: A temperature limited fed-batch (TLFB) technique is described and used for Pichia pastoris Mut+ strain cultures and compared with the traditional methanol limited fed-batch (MLFB) technique. A recombinant fusion protein composed of a cellulose-binding module (CBM) from Neocallimastix patriciarum cellulase 6A and lipase B from Candida antarctica (CALB), was produced and secreted by this strain. RESULTS: A protein concentration of about 1 g L-1 was produced in the MLFB process… CONTINUE READING

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