Temperature influence on the dynamic binding capacity of a monolithic ion-exchange column.

@article{Miheli2003TemperatureIO,
  title={Temperature influence on the dynamic binding capacity of a monolithic ion-exchange column.},
  author={Igor Miheli{\vc} and Ale{\vs} Podgornik and Tine Koloini},
  journal={Journal of chromatography. A},
  year={2003},
  volume={987 1-2},
  pages={159-68}
}
This work investigates the influence of temperature on the binding capacity of bovine serum albumin (BSA), soybean trypsin inhibitor and L-glutamic acid to a CIM (DEAE) weak anion-exchange disk monolithic column. The binding capacity was determined experimentally under dynamic conditions using frontal analysis. The effect on the dynamic binding capacity of dimers present in the BSA solution has been evaluated and a closed-loop frontal analysis was used to determine the equilibrium binding… CONTINUE READING