Temperature-dependent helix-coil transition of an alanine based peptide.

  title={Temperature-dependent helix-coil transition of an alanine based peptide.},
  author={C Y Huang and Jason W Klemke and Zelleka Getahun and William F. DeGrado and Feng Gai},
  journal={Journal of the American Chemical Society},
  volume={123 38},
The helix-coil transition of a synthetic alpha-helical peptide (the D-Arg peptide), Ac-YGG(KAAAA)(3)-CO-D-Arg-CONH(2), was studied by static far-UV circular dichroism (CD) and time-resolved infrared spectroscopy coupled with the laser-induced temperature-jump technique for rapid relaxation initiation. Equilibrium thermal unfolding measurements of the D-Arg peptide monitored by CD spectroscopy reveal an apparent two-state helix-coil transition, with a thermal melting temperature around 10… CONTINUE READING