Temperature dependence of the inhibitory effects of orthovanadate on shortening velocity in fast skeletal muscle.

@article{Pate1994TemperatureDO,
  title={Temperature dependence of the inhibitory effects of orthovanadate on shortening velocity in fast skeletal muscle.},
  author={Edward Pate and Gerard J Wilson and Munsif Bhimani and Roger Cooke},
  journal={Biophysical journal},
  year={1994},
  volume={66 5},
  pages={1554-62}
}
We have investigated the effects of the orthophosphate (P(i)) analog orthovanadate (Vi) on maximum shortening velocity (Vmax) in activated, chemically skinned, vertebrate skeletal muscle fibers. Using new "temperature-jump" protocols, reproducible data can be obtained from activated fibers at high temperatures, and we have examined the effect of increased [Vi] on Vmax for temperatures in the range 5-30 degrees C. We find that for temperatures < or = 20 degrees C, increasing [Vi] inhibits Vmax… CONTINUE READING