Temperature and quenching studies of fluorescence polarization detection of DNA hybridization.

@article{Kumke1997TemperatureAQ,
  title={Temperature and quenching studies of fluorescence polarization detection of DNA hybridization.},
  author={Michael U Kumke and Lijin Shu and Linda B. McGown and G. Terrance Walker and J Bruce Pitner and Cullen Linn},
  journal={Analytical chemistry},
  year={1997},
  volume={69 3},
  pages={500-6}
}
The effects of temperature and collisional quenching on fluorescence polarization detection of DNA hybridization were studied using measurements of fluorescence intensity and anisotropy and the dynamic decay of these properties. Three different tethers, 3, 6, and 12 carbons in length, were used to attach fluorescein label to the 5' end of the 33-base oligomers. Perrin plots showed that the effective rotating volume decreases with increasing tether length and approximately doubles upon… CONTINUE READING