Temperature- and flow-enhanced detection specificity of mutated DNA against the wild type with reporter microspheres.

  title={Temperature- and flow-enhanced detection specificity of mutated DNA against the wild type with reporter microspheres.},
  author={Ceyhun E. Kirimli and Wei-Heng Shih and Wan Y. Shih},
  journal={The Analyst},
  volume={138 20},
Detection of mutated (MT) deoxyribonucleic acid (DNA) amongst the wild type (WT) requires the probe DNA (pDNA) that is complementary to the MT to discriminate the WT by one or two nucleotide mismatches. Traditionally this is achieved by raising the temperature to above the melting temperature (Tm) of the WT (TWT) but below that of the MT (TMT). However, a raised temperature is also accompanied by a weakened binding of the MT to the pDNA which can reduce the detection sensitivity. In this study… 
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