Telomere measurement by quantitative PCR.

@article{Cawthon2002TelomereMB,
  title={Telomere measurement by quantitative PCR.},
  author={Richard M. Cawthon},
  journal={Nucleic acids research},
  year={2002},
  volume={30 10},
  pages={
          e47
        }
}
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the… CONTINUE READING

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