Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases alpha and delta.

@article{Diede1999TelomerasemediatedTA,
  title={Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases alpha and delta.},
  author={Scott J. Diede and Daniel E Gottschling},
  journal={Cell},
  year={1999},
  volume={99 7},
  pages={723-33}
}
To better understand the requirements for telomerase-mediated telomere addition in vivo, we developed an assay in S. cerevisiae that creates a chromosome end immediately adjacent to a short telomeric DNA tract. The de novo end acts as a telomere: it is protected from degradation in a CDC13-dependent manner, telomeric sequences are added efficiently, and addition occurs at a faster rate in mutant strains that have long telomeres. Telomere addition was detected in M phase arrested cells, which… CONTINUE READING
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