Telomerase activation in human fibroblasts during escape from crisis.

@article{Montalto1999TelomeraseAI,
  title={Telomerase activation in human fibroblasts during escape from crisis.},
  author={Michael C. Montalto and Jennifer S. Phillips and F. Andrew Ray},
  journal={Journal of cellular physiology},
  year={1999},
  volume={180 1},
  pages={46-52}
}
The immortalization of human diploid fibroblasts requires the circumvention of both the senescence (M1) and crisis (M2) mechanisms of growth control. Cells expressing the SV40 T antigen virtually always bypass senescence, but only rarely escape crisis. The low frequency of this latter event indicates that cellular mutations are necessary to escape crisis. Thirteen subpopulations of T antigen-expressing human fibroblasts were cultured into crisis. Colonies that appeared to resume growth were… CONTINUE READING