Techniques to improve detection and analysis of extracellular vesicles using flow cytometry.

@article{Inglis2015TechniquesTI,
  title={Techniques to improve detection and analysis of extracellular vesicles using flow cytometry.},
  author={Heather C. Inglis and Ali Danesh and Avani Shah and Jacques R Lacroix and Philip C Spinella and Phillip J Norris},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2015},
  volume={87 11},
  pages={
          1052-63
        }
}
Extracellular vesicles (EVs) range in size from 50 nm to 1 µm. Flow cytometry (FCM) is the most commonly used method for analyzing EVs; however, accurate characterization of EVs remains challenging due to their small size and lack of discrete positive populations. Here we report the use of optimization techniques that are especially well-suited for analyzing EVs from a high volume of clinical samples. Utilizing a two pronged approach that included 1) pre-filtration of antibodies to remove… CONTINUE READING

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