Techniques of skinned cardiac cells and of isolated cardiac fibers with disrupted sarcolemmas with reference to the effects of catecholamines and of caffeine.

@article{Fabiato1976TechniquesOS,
  title={Techniques of skinned cardiac cells and of isolated cardiac fibers with disrupted sarcolemmas with reference to the effects of catecholamines and of caffeine.},
  author={Alexandre Fabiato and Françoise Fabiato},
  journal={Recent advances in studies on cardiac structure and metabolism},
  year={1976},
  volume={9},
  pages={
          1-94
        }
}
Single cardiac cells with disrupted sarcolemmas were obtained by homogenization in low free [Ca2+] medium. Skinned cardiac cells were obtained by microdissection of remaining pieces of sarcolemmas. In both preparations the buffers bathing the cells were in contact with the structures controlling activation: myofilaments, sarcoplasmic reticulum, and mitochondria. Tension was recorded with a highly sensitive force transducer. The use of either high or low total [EGTA] permitted the study of the… CONTINUE READING

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