Techniques for culture and assessment of limbal stem cell grafts.

  title={Techniques for culture and assessment of limbal stem cell grafts.},
  author={Enzo Di Iorio and Stefano Ferrari and Adriano Fasolo and Elisabetta B{\"o}hm and Diego Ponzin and Vanessa Barbaro},
  journal={The ocular surface},
  volume={8 3},
The therapeutic use of limbal cultures for the permanent regeneration of corneal epithelium in patients with limbal stem cell deficiency (LSCD) has been reported in many studies. According to the guidelines for good manufacturing practice (GMP), strictly regulated procedures and stringent quality control tests are now required to manipulate stem cells as "medicinal products" and make engraftment safer and eventually more successful. This paper describes techniques for optimal preparation of… CONTINUE READING


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advances in corneal surgery and cell therapy: challenges and perspectives for the eye banks

S ferrari, V Barbaro, e Di Iorio
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