Technique for large scale isolation of Discula umbrinella and other foliar endophytic fungi from Quercus species

@article{Cohen1999TechniqueFL,
  title={Technique for large scale isolation of Discula umbrinella and other foliar endophytic fungi from Quercus species},
  author={Susan D. Cohen},
  journal={Mycologia},
  year={1999},
  volume={91},
  pages={917-922}
}
This paper describes a useful large scale technique suitable for isolation of Discula umbrinella and other foliar endophytic fungi. This technique provides an efficient means to collect large numbers of fungal isolates necessary for fungal ecology and genetic variation studies. Also, this method is easily modified for isolation of other fungi residing within a leaf. An oversized vacuum filtration unit was built to accomodate large numbers of leaf discs and all sterilization procedures were… Expand
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References

SHOWING 1-9 OF 9 REFERENCES
Preserving cultures of wood-decaying Basidiomycotina using sterile distilled water in cryovials
TLDR
This study reports on the use of the method of storing fungal cultures in sterile distilled water in cryovials for long-term fungal storage of wood-decaying Basidiomycotina. Expand
Differentiation of isolates of Discula umbrinella (Teleomorph: Apiognomonia errabunda) from beech, chestnut, and oak using randomly amplified polymorphic DNA markers.
TLDR
Cluster analysis of all arbitrary primed amplified DNA fragments showed that the isolates could be placed into four groups corresponding to their host origin, and the high percentage of private RAPD variants within groups is consistent with low gene flow. Expand
Detection of the endophyte Discula umbrinella in buds and twigs of Fagus sylvatica
TLDR
Endophytic fungi have been isolated from buds and twigs of beech trees collected at four different sites in Switzerland and thalli of D. umbrinella may grow from the twigs into the leaf tissues. Expand
Endophytische Pilze in Blättern und Ästen gesunder und geschädigter Buchen (Fagus sylvatica L.)
TLDR
Endophytic fungi in leaves and twigs of healthy and diseased beech trees (Fagus sylvatica L.) were isolated and it has been hypothesized, that less capacity of water and nutrient uptake of diseased trees is responsible for this effect. Expand
Endophytische Pilze in Blättern und Zweigen von Quercus petraea
TLDR
Surface sterilized leaf and twig samples taken in 1989 from sessile oak (Quercus petraea) in Austria yielded host-specific as well as unspecific ubiquistic endophytes. Expand
Infection studies of Discula quercina, an endophyte of Quercus garryana.
TLDR
Discula quercina is a ubiquitous endophyte in Oregon white oak (Quercus garryana) and which tree parts were infected with this endophytes was determined and how infection levels in leaves varied varied. Expand