Technique for Identifying Y Chromosomes in Human Interphase Nuclei

  title={Technique for Identifying Y Chromosomes in Human Interphase Nuclei},
  author={Peter L Pearson and Martin Bobrow and Canio G. Vosa},
CASPERSSON et al.1,2 have reported the staining of mitotic chromosomes in various organisms using fluorescent acridine derivatives. According to Zech3, the distal portion of the human Y chromosome fluoresces brightly when stained with quinacrine mustard. Vosa4 has observed similar fluorescence using quinacrine dihydro-chloride, which is more easily obtainable. We therefore investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the… 
Analysis of Interphase Nuclei
The presence of certain chromosomes in a cell, particularly the sex chromosomes, can be observed not only during mitosis but also in interphase, and these chromosomes show some pecularities.
Quinacrine Fluorescence in Mammalian Chromosomes
An investigation of the chromosomes of various mammals, including man, is described to see whether quinacrine fluorescence is a universal property of all mammalian Y chromosomes or a reaction specific to man.
Fluorescence staining method for the morphological and structural study of human chromosomes
Very clear pictures of metaphase human chromosomes from peripheral blood cultures have been obtained by way of Acranil (A) staining to evidence the strong fluorescence of the distal half of the long arm of the Y chromosome and the corresponding fluorescent interphase body (IY).
Quinacrine fluorescence of variant and abnormal human Y chromosomes
Quinacrine fluorescence has been used to examine variant and abnormal human Y chromosomes, at interphase and mitosis. The length difference in variant Y chromosomes has been shown to involve the
Fluorescence study of interphase nuclei and double Y chromosomes.
By using interphase nuclei to determine the frequency of double Y-chromosomes by fluorescence, a number of falsely positive results is given, but the procedure is found extremely valuable for screening purposes.
Identification of an Unusual Y Chromosome in YY Mosaicism by Quinacrine Fluorescence
The quinacrine fluorescence technique permitted us to identify the Y chromosome, which in this case is unusually large and not identifiable for certain by other means.
Para-nucleolar Position of the Human Y Chromosome in Interphase Nuclei
It has recently become possible to locate the major portion of the Y chromosomes in interphase nuclei1, and this technique is used to demonstrate that the Y chromosome is spatially associated with the nucleolus.
Fluorescing structures of human metaphase chromosomes. Detection of "Y body".
Ch Chromosomes from several types of plant material and also from Chinese hamsters were investigated in detail by Caspersson and his coworkers and it was shown that fluorescent cross-striations traversing both sister chromatids constitute clear, fully reproducible patterns.
Biological Sciences: Quinacrine Staining of Chromosomes and Evolutionary Studies in Drosophila
It is found that these patterns vary in interesting ways and that they are a characteristic of chromosomes that can be used in studies of evolutionary cytogenetics in much the same way as structural rearrangements17–19.
Chromosomal aneuploidy in human spermatozoa
The results on the frequency of YY gametes in healthy fertile men are in good agreement with the results of other studies: Pearson and Bobrow (1970) found 1.4% spermatids with 2 fluorescent bodies each in testicular tissue of 3 normal men are believed to contain a supernumerary Y chromosome.


A Morphological Distinction between Neurones of the Male and Female, and the Behaviour of the Nucleolar Satellite during Accelerated Nucleoprotein Synthesis
It appears not to be generally known that the sex of a somatic cell as highly differentiated as a neurone may be detected with no more elaborate equipment than a compound microscope following staining of the tissue by the routine Nissl method.
Chemical differentiation along metaphase chromosomes.