Technique for Identifying Y Chromosomes in Human Interphase Nuclei

  title={Technique for Identifying Y Chromosomes in Human Interphase Nuclei},
  author={Peter L Pearson and Martin Bobrow and Canio G. Vosa},
CASPERSSON et al.1,2 have reported the staining of mitotic chromosomes in various organisms using fluorescent acridine derivatives. According to Zech3, the distal portion of the human Y chromosome fluoresces brightly when stained with quinacrine mustard. Vosa4 has observed similar fluorescence using quinacrine dihydro-chloride, which is more easily obtainable. We therefore investigated the possibility of positively identifying male nuclei in interphase by virtue of this staining property of the… 

Diploid and heteroploid human cell lines surveyed for Y chromosome fluorescence.

The distal portion of the Y is intensely brilliant by comparison with other chromosomal segments in the human karyotype and is therefore unambiguously identifiable and translocated Y segments can be recognized because of this property.

Fluorescent staining of the Y chromosome in meiotic stages of the human male.

By the use of this staining reaction, the Y chromosome can be detected in interphase nuclei of lymphocytes, cultured skin fibroblasts and buccal mucosal cells and has also been used to demonstrate that the X chromosome pairs with the short.

Quinacrine Fluorescence in Mammalian Chromosomes

An investigation of the chromosomes of various mammals, including man, is described to see whether quinacrine fluorescence is a universal property of all mammalian Y chromosomes or a reaction specific to man.

Fluorescence staining method for the morphological and structural study of human chromosomes

Very clear pictures of metaphase human chromosomes from peripheral blood cultures have been obtained by way of Acranil (A) staining to evidence the strong fluorescence of the distal half of the long arm of the Y chromosome and the corresponding fluorescent interphase body (IY).

Quinacrine fluorescence of variant and abnormal human Y chromosomes

Quinacrine fluorescence has been used to examine variant and abnormal human Y chromosomes, at interphase and mitosis. The length difference in variant Y chromosomes has been shown to involve the

Bovine chromosomes identified by quinacrine mustard and fluorescence microscopy.

The fluorescence karyotype described is reproducible and can therefore be applied in veterinary cytogenetics, genetic pathology and reproduction research.

Fluorescence study of interphase nuclei and double Y chromosomes.

By using interphase nuclei to determine the frequency of double Y-chromosomes by fluorescence, a number of falsely positive results is given, but the procedure is found extremely valuable for screening purposes.

Identification of an Unusual Y Chromosome in YY Mosaicism by Quinacrine Fluorescence

The quinacrine fluorescence technique permitted us to identify the Y chromosome, which in this case is unusually large and not identifiable for certain by other means.

Para-nucleolar Position of the Human Y Chromosome in Interphase Nuclei

It has recently become possible to locate the major portion of the Y chromosomes in interphase nuclei1, and this technique is used to demonstrate that the Y chromosome is spatially associated with the nucleolus.

Fluorescing structures of human metaphase chromosomes. Detection of "Y body".

Ch Chromosomes from several types of plant material and also from Chinese hamsters were investigated in detail by Caspersson and his coworkers and it was shown that fluorescent cross-striations traversing both sister chromatids constitute clear, fully reproducible patterns.



A Morphological Distinction between Neurones of the Male and Female, and the Behaviour of the Nucleolar Satellite during Accelerated Nucleoprotein Synthesis

It appears not to be generally known that the sex of a somatic cell as highly differentiated as a neurone may be detected with no more elaborate equipment than a compound microscope following staining of the tissue by the routine Nissl method.

Chemical differentiation along metaphase chromosomes.