Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat.

@article{Bereczki1992TechniquedependentVI,
  title={Technique-dependent variations in cerebral microvessel blood volumes and hematocrits in the rat.},
  author={D Bereczki and Lian Kian Wei and V R Acuff and K. Gruber and Ai Tajima and C Patlak and Joseph D. Fenstermacher},
  journal={Journal of applied physiology},
  year={1992},
  volume={73 3},
  pages={918-24}
}
To quantitate small parenchymal microvessel blood volumes in the brain, the distribution spaces of radiolabeled red blood cells (RBC) and serum albumin (RISA) were assessed in rats by different methods of tissue sampling and radioassay. Three minutes after intravenous administration of 55Fe-RBCs and/or 125I-RISA, the rats were decapitated. The brain was either immediately frozen within the skull and later removed (head-frozen group) or rapidly removed from the skull and then frozen (brain… CONTINUE READING
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