Technical improvements in corrosion casting of small specimens: a study on mesonephric tubules and vessels of chicken embryos.
@article{Carretero1993TechnicalII, title={Technical improvements in corrosion casting of small specimens: a study on mesonephric tubules and vessels of chicken embryos.}, author={A. Carretero and H. Ditrich and M. Navarro and H. Splechtna and J. Ruberte}, journal={Scanning microscopy}, year={1993}, volume={7 4}, pages={ 1333-7; discussion 1337-8 } }
The injection technique for corrosion casting of small, embryonic material can be improved by using a "chemical ligature" (cyanoacrylate). With this simple method, leakage of the resin at the injection site is prevented and the mechanical stability of the cannula-vessel coupling is improved. The blood vascular system of chicken embryos as small as Hamburger-Hamilton stage 24 (approximately 4.5 days of incubation) has successfully been injected using this procedure. Corrosion casts of the… CONTINUE READING
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