Technical advance. Double-stranded RNA interferes with gene function at the single-cell level in cereals.

@article{Schweizer2000TechnicalAD,
  title={Technical advance. Double-stranded RNA interferes with gene function at the single-cell level in cereals.},
  author={Patrick Schweizer and Jan Pokorn{\'y} and Paul Schulze-Lefert and R. Dudler},
  journal={The Plant journal : for cell and molecular biology},
  year={2000},
  volume={24 6},
  pages={895-903}
}
Double-stranded RNA (dsRNA) has been shown to specifically interfere with gene function in several organisms including tobacco and the model plant Arabidopsis. Here, we report on rapid and sequence-specific interference of dsRNA with gene function in cereals. Delivery of cognate dsRNA into single epidermal cells of maize, barley or wheat by particle bombardment interfered with the function of co-bombarded UidA (GUS) and TaGLP2a:GFP reporter genes. Cognate dsRNA was also found to specifically… CONTINUE READING
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