Technical advance: the DNA-binding activity of gal4 is inhibited by methylation of the gal4 binding site in plant chromatin.

@article{Glweiler2000TechnicalAT,
  title={Technical advance: the DNA-binding activity of gal4 is inhibited by methylation of the gal4 binding site in plant chromatin.},
  author={L G{\"a}lweiler and R Steven Conlan and Pavel Mader and Klaus Palme and Ian Moore},
  journal={The Plant journal : for cell and molecular biology},
  year={2000},
  volume={23 1},
  pages={143-57}
}
Derivatives of the Saccharomyces cerevisiae transcription factor Gal4 which act as effective transcription activators in yeast, Drosophila, mammalian cells and plant protoplasts are shown to direct expression from a GUS reporter construct when expressed in transgenic tobacco. However, in comparison to 35S-GUS controls, Gal4-mediated expression of the reporter gene was relatively weak and extremely variable. GUS expression was lost as plants matured and it was almost undetectable in most of… CONTINUE READING

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