Technetium-99m labeling of human monocytes for chemotactic studies.

  title={Technetium-99m labeling of human monocytes for chemotactic studies.},
  author={Cynthia K Papierniak and R E Bourey and Roberto R. Kretschmer and Samuel P. Gotoff and L G Colombetti},
  journal={Journal of nuclear medicine : official publication, Society of Nuclear Medicine},
  volume={17 11},
Although the current use of 51Cr for the evaluation of monocyte chemotaxis has yielded encouraging results, certain properties of this radionuclide leave room for improvement. Technetium-99m was evaluated as an alternative label for this purpose. A cell loss of 50% was found, but the recovered cells showed excellent viability and function. Chemotaxis was measured using a modified Boyden's chamber and a lymphocyte-derived chemotactic factor. The chemotactic properties of 99mTc-labeled human… CONTINUE READING