Tcl transposition and mutator activity in a Bristol strain of Caenorhabditis elegans

@article{Babity2004TclTA,
  title={Tcl transposition and mutator activity in a Bristol strain of Caenorhabditis elegans},
  author={J. M. Babity and T. Starr and A. Rose},
  journal={Molecular and General Genetics MGG},
  year={2004},
  volume={222},
  pages={65-70}
}
SummaryIn most strains of Caenorhabditis elegans with a low copy number of Tc1 transposable elements, germline transposition is rare or undetectable. We have observed low-level Tel transposition in the genome of the C. elegans var. Bristol strain KR579 (unc-13[e51]) resulting in an increase in Tc1 copy number and subsequent mutator activity. Examination of genomic blots from KR579 and KR579derived strains revealed that more Tc1-hybridizing bands were present than in other Bristol strains. A… Expand
Strain evolution in Caenorhabditis elegans: Transposable elements as markers of interstrain evolutionary history
TLDR
A composite evolutionary tree is proposed, based on the locations and numbers of Tc1 elements in these strains, which is consistent with a four-branch intraspecific tree deduced previously by maximum-parsimony analyses of mitochondrial sequence changes; it also serves to elucidate the evolutionary history of transposon mobility. Expand
Population frequencies of transposable elements in selfing and outcrossing Caenorhabditis nematodes.
TLDR
It is shown that transposons are less polymorphic and segregate at higher frequencies in C. elegans compared with C. remanei, consistent with a reduced efficacy of natural selection against TEs in self-fertilizing populations, but may be explained by non-equilibrium TE dynamics. Expand
Genomic sequence of a mutant strain of Caenorhabditis elegans with an altered recombination pattern
TLDR
The major phenotype of the Rec-1 strain is an alteration in the preferred position of the meiotic recombination event with no other significant phenotypic consequences. Expand
Natural Variation and Copulatory Plug Formation in Caenorhabditis eleguns
Most of the available natural isolates of the nematode Caenorhabditis elegans have been examined and compared with the standard laboratory wild type (Bristol N2). Molecular markers, in particularExpand
Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes
TLDR
The results suggest that the blurry promoter could be a shared feature of the members of the Tc1/mariner superfamily with possible evolutionary and biotechnological implications. Expand
The effects of breeding systems on genetic architecture
TLDR
A theoretical framework for analyzing TE dynamics under asexual reproduction is provided and results show that, in an infinite asexual population, an equilibrium in copy number is achieved if no element excision is possible, but that all TEs are eliminated if there is some excision. Expand

References

SHOWING 1-10 OF 35 REFERENCES
Structural analysis of Tc1 elements in Caenorhabditis elegans var. Bristol (strain N2).
The transposable element Tc1 in the genome of Caenorhabditis elegans var. Bristol strain N2 is very stable. In order to investigate possible causes of Tc1 immobility in this strain 17 individualExpand
Analysis of a mutator activity necessary for germline transposition and excision of Tc1 transposable elements in Caenorhabditis elegans.
TLDR
The single mutator-harboring strains with low Tc1 copy number generated in this study should be useful in investigations of the molecular basis of mutator activity, and three possible candidates were identified: none was larger than 1.6 kb. Expand
Transposition of Tcl in the nematode Caenorhabditis elegans
We have identified a strain of Caenorhabditis elegans in which the transposable element Tcl is genetically active. Most spontaneous mutations affecting the unc-54 myosin heavy chain gene of C. ekgansExpand
Transposition of Tc1 in the nematode Caenorhabditis elegans.
  • D. Eide, P. Anderson
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1985
We have identified a strain of Caenorhabditis elegans in which the transposable element Tc1 is genetically active. Most spontaneous mutations affecting the unc-54 myosin heavy chain gene of C.Expand
Isolation of the closed circular form of the transposable element Tc1 in Caenorhabditis elegans
TLDR
The recovery of extrachromosomal linear and closed circular copies of Tc1 from the Bergerac strain is described, presumably a result of T c1 excision. Expand
Somatic excision of transposable element Tc1 from the Bristol genome of Caenorhabditis elegans.
TLDR
It is suggested that Bristol Tc1 elements have the ability to transpose but that transposition is heavily repressed in the gonadal tissue. Expand
Evidence for a transposon in caenorhabditis elegans
TLDR
The C. elegans genome contains a 1.7 kb repeated DNA sequence (Tc1) that is present in different numbers in various strains, and this structure was analyzed by the cloning of a single Tc1 element from Bergerac and the comparison with homologous genomic sequences in Bristol and Bergerac. Expand
Insertion and excision of Caenorhabditis elegans transposable element Tc1.
TLDR
The genetic properties of Tc1 excision, combined with the DNA sequences of the resulting unc-54 alleles, demonstrated that excision was dependent on T c1 transposition functions in both germ line and somatic cells. Expand
Molecular cloning of the muscle gene unc-22 in Caenorhabditis elegans by Tc1 transposon tagging.
TLDR
The availability of a transposon-based mutator system should aid in the cloning of additional genes in C. elegans, and the particular properties of this Tc1 system may provide information about the control of transposable element activity more generally. Expand
Extrachromosomal copies of transposon Tc1 in the nematode Caenorhabditis elegans.
  • K. Ruan, S. W. Emmons
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1984
TLDR
The suggestion is made that these extrachromosomal transposable elements are the products of excision events known to be occurring at high frequency in somatic cells in Bergerac. Expand
...
1
2
3
4
...