Tb(III) and Eu(III) as fluorescent probes to investigate the metal-binding sites of trichosanthin.

@article{Zhang1993TbIIIAE,
  title={Tb(III) and Eu(III) as fluorescent probes to investigate the metal-binding sites of trichosanthin.},
  author={Ying-jiu Zhang and Wen Li and Quan Hao and Guixue Yu and Qian Li and Qingwei Yao},
  journal={Biochemical and biophysical research communications},
  year={1993},
  volume={197 2},
  pages={
          407-14
        }
}
Tb(III) and Eu(III) ions were used as fluorescent probes in the study of the trichosanthin (TCS). The fluorescence of Tb(III) was increased considerably when bound to TCS to replace the Ca(II) ions. The nonradiative energy transfer from fluorescent tryptophan (Trp) residues to the bound Tb(III) or Eu(III) took place. From a Foster d-d nonradiative energy transfer mechanism, it was obtained that the average distance between the bound Tb(III) and the Trp residue is 1.27nm. The results indicate… CONTINUE READING