Taxonomic Investigations on Expressed and Cryptic Phospho-β-glucosidases in Enterobacteriaceae

  title={Taxonomic Investigations on Expressed and Cryptic Phospho-$\beta$-glucosidases in Enterobacteriaceae},
  author={S. Schaefler and A. Malamy},
  journal={Journal of Bacteriology},
  pages={422 - 433}
In the Enterobacteriaceae, β-glucosides are catabolized by a complex system formed of three permeases, with partly overlapping substrate specificities, and two hydrolytic enzymes, phospho-β-glucosidase A and B, which hydrolyze only phosphorylated β-glucosides. Some Enterobacteriaceae such as Klebsiella-Aerobacter (Enterobacter) possess the complete system; others possess only parts of it or may have a cryptic phospho-β-glucosidase activity without permease activity. A screening test applied to… 
Characterization of a β-Glucoside Operon (bgc) Prevalent in Septicemic and Uropathogenic Escherichia coli Strains
The prevalence of bgc in strains of phylogenetic group B2, representing mainly extraintestinal pathogens, while it is rare among commensal E. coli strains was demonstrated and bioinformatic analyses demonstrated that homologs of the bgc genes exist in the enterobacterial Klebsiella, Enterobacter, and Citrobacter spp.
Phenotypic variability of beta-glucoside utilization and its correlation to pathogenesis process in a few enteric bacteria.
  • A. Kharat
  • Medicine, Biology
    FEMS microbiology letters
  • 2001
The genomic hybridization of selected Enterobacteriaceae members with the Escherichia coli bgl and cel genes resulted in detection of a complete homolog of the bgland cel operons in Shigella sonnei, a member that is evolutionarily closest to E. coli.
Analysis of the Erwinia chrysanthemi arb genes, which mediate metabolism of aromatic beta-glucosides
The isolation of the arb genes is reported, which permit fermentation of the aromatic beta-glucosides only and underline the resemblance between the naturally expressed E. chrysanthemi arbGFB and the cryptic E. coli bgl operon expression.
Analysis of the beta-glucoside utilization (bgl) genes of Shigella sonnei: evolutionary implications for their maintenance in a cryptic state.
The results presented here indicate that the homologue of the cryptic bgl operon of E. coli is conserved in Shigella sonnei and is the primary system involved in beta-glucoside utilization in the organism.
Nucleotide sequences of the arb genes, which control beta-glucoside utilization in Erwinia chrysanthemi: comparison with the Escherichia coli bgl operon and evidence for a new beta-glycohydrolase family including enzymes from eubacteria, archeabacteria, and humans
A genetic analysis of the E. chrysanthemi arb genes revealed the conservation of two regions which, in the latter, are known to terminate transcription under noninducing conditions and be the target of the BglG transcriptional antiterminator under inducing conditions, and predicted that domain II of the human lactate-phlorizin hydrolase, which is involved in lactose intolerance, is catalytically nonactive.
Diverse pathways for salicin utilization in Shigella sonnei and Escherichia coli carrying an impaired bgl operon
The pathway to achieve salicin utilization is diverse in Shigella sonnei and E. coli strain tree; however, both show cooperation between two silent genetic systems to achieve a new metabolic capability under selection.
Genetic determination of the constitutive biosynthesis of phospho- -glucosidase A in Escherichia coli K-12.
Escherichia coli wild-type cells form constitutively the enzyme phospho-β-glucosidase A, which has a high affinity for phosphorylated aromatic β-glucosides and a low affinity for phosphorylated
Utilization of cellobiose and other β-D-glucosides in Agrobacterium tumefaciens
Abstract Agrobacterium tumefaciens strain C58 was able to utilize carbon from cellobiose and some other β-D-glucosides as efficiently as from glucose. β-D-glucoside utilization was partially
Isolation and characterisation of an aryl-β-D-glucoside uptake and utilisation system (abg) from the gram-positive ruminal Clostridium species C. longisporum
Although it was not possible to demonstrate any function for AbgG, a promoter 5′ to the abgG gene was identified which was responsible for expression of the downstream genes.
Regulation of gene expression: Cryptic β-glucoside (bgl) operon of Escherichia coli as a paradigm
  • D. Harwani
  • Biology, Medicine
    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
  • 2014
The bgl operon of Escherichia coli, involved in the uptake and utilization of aromatic β-glucosides salicin and arbutin, is maintained in a silent state in the wild type organism by the presence of structural elements in the regulatory region.


Phospho-β-glucosidases and β-Glucoside Permeases in Streptococcus, Bacillus, and Staphylococcus
With the exception of four strains isolated in Japan, all strains of B. subtilis tested possess an inducible phospho-β-glucosidase activity, β-methyl glucoside, cellobiose, and TPG acting as inducers, which could serve as an additional criterion for their differentiation from S. albus.
Inducible System for the Utilization of β-Glucosides in Escherichia coli II. Description of Mutant Types and Genetic Analysis
Interrupted mating experiments and cotransduction with P1kc phage showed that the genetic determinants of the beta-glucoside system map between the pyrE and ile loci.
Inducible System for the Utilization of β-Glucosides in Escherichia coli I. Active Transport and Utilization of β-Glucosides
Autoradiography shows that TEG is accumulated by the β-glucoside permease and glucose permease in two different forms, and it is assumed that phosphorylated alkyl and thioalkyl β- glucosides inhibit the induction, and that this inhibition is responsible for the induction lag.
On the Evolution of the Lactose Utilization Gene System in Enteric Bacteria
The loss of permease has played the key role in the evolution of the lac region by rendering the galactosidase inoperative in lactose utilization and has permitted the accumulation of a variety of mutations within the z gene.
Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles.
Transduction of the lac + property by phage P1 among strains of Escherichia coli K12 and Shigella dysenteriae Sh has been studied and a variety of P1 dl elements is postulated to account for the properties of different Sh lac v transductant strains.
Replacement of a Phosphoenolpyruvate-dependent Phosphotransferase by a Nicotinamide Adenine Dinucleotide-linked Dehydrogenase for the Utilization of Mannitol
Mannitol is dissimilated by Aerobacter aerogenes via an inducible pathway initiated by a phosphotransferase system dependent upon phosphoenolpyruvate as the phosphoryl donor. A mutational block in
Certain biochemical properties of auxotrophicl mutants of Escherichia coli with specific growth requirements for most of the known water-soluble vitamins, as well as of others responding to methionine but not to B,2 are described.
It is doubtful whether a general work of this calibre should contain even brief accounts and exiguous illustrations of operations, but the editors have produced a magnunm opus which for many years will be indispensable to those who practise obstetrics and gynaecology.
Study of Microbial Evolution through Loss of Biosynthetic Functions: Establishment of “Defective” Mutants
On appropriate media, mutants which have lost a biosynthetic function may exhibit a selective advantage over parental strains. Is this how “parasitism” became established ?
S-Glucoside permeases and phospho ,-glucosidases in Aerobacter aerogenes: relationship with cryptic phospho ,j-glucosidases in Enterobacteriaceae
  • Proc. Nat. Acad. Sci. U.S.A
  • 1968