Taxol-dependent mutants of Chinese hamster ovary cells with alterations in alpha- and beta-tubulin

@article{Schibler1986TaxoldependentMO,
  title={Taxol-dependent mutants of Chinese hamster ovary cells with alterations in alpha- and beta-tubulin},
  author={Matthew J. Schibler and Francisco Romero Cabral},
  journal={The Journal of Cell Biology},
  year={1986},
  volume={102},
  pages={1522 - 1531}
}
Chinese hamster ovary cell mutants resistant to the microtubule stabilizing drug taxol were isolated in a single step. Of these 139 drug-resistant mutants, 59 exhibit an absolute requirement for taxol for normal growth and division, 13 have a partial requirement, and 69 grow normally without the drug. Two-dimensional gel analysis of whole cell proteins reveal "extra" spots representing altered tubulins in 13 of the mutants. Six of these have an altered alpha-tubulin and seven have an altered… CONTINUE READING