Targets of the cyclin-dependent kinase Cdk1

@article{Ubersax2003TargetsOT,
  title={Targets of the cyclin-dependent kinase Cdk1},
  author={Jeffrey A. Ubersax and Erika L. Woodbury and Phuong N. Quang and Maria T.Z. Paraz and Justin D. Blethrow and Kavita Shah and Kevan M. Shokat and David O. Morgan},
  journal={Nature},
  year={2003},
  volume={425},
  pages={859-864}
}
The events of cell reproduction are governed by oscillations in the activities of cyclin-dependent kinases (Cdks). Cdks control the cell cycle by catalysing the transfer of phosphate from ATP to specific protein substrates. Despite their importance in cell-cycle control, few Cdk substrates have been identified. Here, we screened a budding yeast proteomic library for proteins that are directly phosphorylated by Cdk1 in whole-cell extracts. We identified about 200 Cdk1 substrates, several of… 
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TLDR
A novel computational procedure to predict substrates of the cyclin-dependent kinase Cdc28 (Cdk1) in the Saccharomyces cerevisiae by defining the local sequence motifs that represent the CDC28 phosphorylation sites and subsequently model clustering of these motifs within the protein sequences.
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References

SHOWING 1-10 OF 54 REFERENCES
Cyclin-dependent kinases: engines, clocks, and microprocessors.
  • D. Morgan
  • Biology, Medicine
    Annual review of cell and developmental biology
  • 1997
TLDR
This work has shown that Cdk activity is governed by a complex network of regulatory subunits and phosphorylation events whose precise effects on Cdk conformation have been revealed by recent crystallographic studies.
Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex.
TLDR
The mutual inhibition between APC and CDKs explains how cells suppress mitotic CDK activity during G1 and then establish a period with elevated kinase activity from S phase until anaphase.
Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14
TLDR
It is found that Hct1 was phosphorylated in vivo at multiple CDK consensus sites during cell cycle stages when activity of the cyclin-dependent kinase Cdc28 is high and APC activity is low, and phosphorylation abolished the ability of HCT1 to activate the APC in vitro.
Phosphorylation of Sic1p by G1 Cdk required for its degradation and entry into S phase.
TLDR
A molecular model of how phosphorylation and proteolysis cooperate to bring about the G1/S transition in budding yeast is suggested.
S-Cdk-dependent phosphorylation of Sld2 essential for chromosomal DNA replication in budding yeast
TLDR
Sld2, one of the replication proteins of budding yeast, is phosphorylated in S phase in an S-Cdk-dependent manner, and mutant Sld2 lacking all the preferred Cdk phosphorylation sites (All-A) is defective in chromosomal DNA replication.
Pheromone-Dependent G1 Cell Cycle Arrest Requires Far1 Phosphorylation, but May Not Involve Inhibition of Cdc28-Cln2 Kinase, In Vivo
TLDR
A systematic mutational analysis of potential MAP kinase and Cdk recognition motifs found two putative phosphorylation sites that strongly affect Far1 behavior, causing enhanced sensitivity to pheromone and suggesting unconventional inhibitory mechanisms of Far1.
Ubiquitination of the G1 cyclin Cln2p by a Cdc34p‐dependent pathway.
TLDR
The results provide a molecular framework for G1 cyclin instability and suggest that a multicomponent, regulated pathway specifies the selective ubiquitination of G 1 cyclins.
Cyclin-dependent kinases prevent DNA re-replication through multiple mechanisms
TLDR
It is shown that B-type CDKs in Saccharomyces cerevisiae prevent re-initiation through multiple overlapping mechanisms, including phosphorylation of the origin recognition complex (ORC), downregulation of Cdc6 activity, and nuclear exclusion of the Mcm2-7 complex.
A chemical switch for inhibitor-sensitive alleles of any protein kinase
TLDR
A chemical genetic strategy for sensitizing protein kinases to cell-permeable molecules that do not inhibit wild-type kinases is described, allowing for rapid functional characterization of members of this important gene family.
...
1
2
3
4
5
...