Targets for pSAM2 integrase-mediated site-specific integration in the Mycobacterium smegmatis chromosome.

Abstract

An improved integrative cassette from plasmid pSAM2 has been constructed containing plasmid int and attP genes but excluding the xis gene, which should results in increased stability by suppression of the excision reaction. This cassette was included in both suicide and thermosensitive plasmids and used for integration in Mycobacterium smegmatis. Suicide… (More)

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