Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast

@article{Heitman1991TargetsFC,
  title={Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast},
  author={Joseph Heitman and NR Movva and MN Hall},
  journal={Science},
  year={1991},
  volume={253},
  pages={905 - 909}
}
FK506 and rapamycin are related immunosuppressive compounds that block helper T cell activation by interfering with signal transduction. In vitro, both drugs bind and inhibit the FK506-binding protein (FKBP) proline rotamase. Saccharomyces cerevisiae cells treated with rapamycin irreversibly arrested in the G1 phase of the cell cycle. An FKBP-rapamycin complex is concluded to be the toxic agent because (i) strains that lack FKBP proline rotamase, encoded by FPR1, were viable and fully resistant… Expand
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